OxiSelect™ In Vitro ROS/RNS Assay Kit (Green Fluorescence) - 96 Tests

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    OxiSelect In Vitro ROS/RNS Assay Kit (Green Fluorescence), 96T

    Product details

    The OxiSelect™ In Vitro ROS/RNS Assay provides a sensitive method to detect total reactive oxygen species (ROS) plus reactive nitrogen species (RNS) in a wide variety of sample types. This assay employs a proprietary fluorogenic probe, DCFH-DiOxyQ; the probe is primed with a dequenching reagent to the highly reactive DCFH form. In the presence of ROS and RNS, the DCFH is rapidly oxidized to the highly fluorescent DCF.

     OxiSelect In Vitro ROS/RNS Assay Kit (Green Fluorescence), 96T

    Assay Principle.

     OxiSelect In Vitro ROS/RNS Assay Kit (Green Fluorescence), 96T

    Hydrogen Peroxide Standard Curve.

    OxiSelect In Vitro ROS/RNS Assay Kit (Green Fluorescence), 96T

    Detection of Various Free Radical Species. DCF fluorescence curves for AAPH (peroxyl radical generator), SIN-1 (peroxynitrite generator), and SNP (nitric oxide generator).