Histone H3K9me3 (H3K9 Trimethyl) Polyclonal Antibody - 50 µl

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    Histone H3K9me3 (H3K9 Trimethyl) Polyclonal Antibody - 50 µl

    Background
    Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin (1). The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (2-5). These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression (6). In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20 (4,7). Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3). Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (8-10). Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during anaphase (11).

    Description
    Histone H3K9me3 (H3K9 Trimethyl) Polyclonal Antibody. Unconjugated. Raised in: Rabbit.

    Formulation
    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

    Specificity
    Broad Range, Mouse, Rat, Human

    Isotype
    IgG

    Uniprot ID
    Q16695

    Purification
    Affinity Purified

    Immunogen
    A synthetic trimethylated peptide around K9 of human histone H3 (NP_003520.1)

    Storage
    Shipped at 4°C. Upon receipt, store at -20°C. Avoid repeated freeze.

    Alternative Names
    H3K9me3 antibody; H3K9m3 antibody; HIST1H3J; H3/j; H3FJ; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/I; Histone H3/j; Histone H3/k; Histone H3/l; HIST3H3

    Application
    DB, WB, IHC, IF, ChIP; Recommended dilution: DB 1:500 - 1:2000, WB 1:500 - 1:2000, IHC 1:50 - 1:200, IF 1:50 - 1:200, ChIP 1:50 - 1:200