Description: Recombinant, purified, tag-free Staphylococcus aureus Panton-Valentine Leukocidin (PVL) S subunit (LukS-PV). The theoretical molecular weight of the protein is 32,465 Daltons.
Storage: 2-3 weeks at -20°C, -80°C long term
Size: 100 µg of protein is supplied in PBS plus 5% glycerol at a concentration of 2.607 mg/mL. Protein demonstrates a molecular weight of approximately 35 kDa.
Relevance: This protein may be used in functional PVL toxicity assays in combination with LukF-PV, or as a control protein in ELISA assays or Western blotting when detecting LukS in PVL (+) strains of S. aureus.
Recommended Dilutions: ELISA: Assay-dependent dilution.
WB: Assay-dependent dilution; internal QC demonstrates detection of 50 ng of LukS-PV protein when detected with anti-PVL LukS polyclonal antibody. PVL
Cytotoxicity assay: Cytotoxicity can be detected in human
neutrophils when used in combination with LukF-PV in a
concentration range of 1-100 nM.