Promoter: T5
Replicon: ColE1 ori
Terminator: lambda t0 Terminator
Plasmid classification: Escherichia coli plasmids; large intestine expression plasmids; pQE series plasmids
Plasmid size: 3461bp
Plasmid label: N-His
Prokaryotic resistance: ampicillin Amp (100 u g/ml)
Cloned strains of Escherichia coli, DH5 A and other Escherichia coli
Culture conditions: 37 centigrade, aerobic, LB
Expression host: M15
Culture conditions: 37 centigrade, aerobic, LB
Inducement: IPTG or lactose and its analogues
5'sequencing primers: pQE30-F (TGAGCGGATAACAATTTCAC)
3'sequencing primers: pQE -R (GTTCTGAGGTCATTACTGG)