Human Holo TC (Holotranscobalamin), Active vit. B-12 ELISA Kit - 96 wells plate

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    Product name:Human Holo TC(Holotranscobalamin) ELISA Kit
    Reactivity:Human
    Alternative Names:Holo-TC
    Assay Type:Competitive Inhibition
    Sensitivity:0.047 ng/mL
    Standard:10 ng/mL
    Detection Range:0.16-10 ng/mL
    Sample Type:serum, plasma and other biological fluids
    Assay Length:2.5h
    Research Area:Metabolic pathway;Nutrition metabolism;
    Test principle:This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human Holo TC protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Holo TC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Holo TC in the samples is then determined by comparing the OD of the samples to the standard curve.


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