This ELISA kit is for quantification of IL-1β in salmon. This is a quick ELISA assay that
reduces time to 50% compared to the conventional method, and the entire assay only takes 3 hours. This assay employs the quantitative sandwich enzyme immunoassay technique and uses biotin-streptavidin chemistry to improve the performance of the assays. An antibody specific for Salmon IL-1β has been pre-coated onto a microplate. Standards and samples are pipetted into the
wells and any IL-1β present is bound by the immobilized antibody. After washing away any unbound substances, a detection antibody specific for Salmon IL-1β is added to the wells. Following wash to remove any unbound antibody reagent, a detection reagent is added. After intensive wash a substrate solution is added to the wells and color develops in proportion to the amount of IL-1β bound in the initial step. The color development is stopped, and the intensity of the color is measured.